Structural phenotyping of stem cell-derived cardiomyocytes

Francesco Silvio Pasqualini; Sean Paul Sheehy; Ashutosh Agarwal; Yvonne Aratyn-Schaus; Kevin Kit Parker

doi:10.1016/j.stemcr.2015.01.020

Back

Structural phenotyping of stem cell-derived cardiomyocytes

Journal article

Open access

Peer reviewed

Structural phenotyping of stem cell-derived cardiomyocytes

Francesco Silvio Pasqualini, Sean Paul Sheehy, Ashutosh Agarwal, Yvonne Aratyn-Schaus and Kevin Kit Parker

Stem cell reports, Vol.4(3), pp.340-347

2015-03-10

DOI: https://doi.org/10.1016/j.stemcr.2015.01.020

PMID: 25733020

Abstract

Myocytes, Cardiac - cytology

Humans

Rats

Stem Cells - cytology

Biometry - methods

Machine Learning

Phenotype

Algorithms

Animals

Cytoskeleton - metabolism

Myocytes, Cardiac - metabolism

Cell Differentiation

Mice

Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein α-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.

Files and links (1)

url

https://doi.org/10.1016/j.stemcr.2015.01.020View

Published (Version of record) Open

Metrics

11 Record Views

70 Times Cited - Web of Science

See more details

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Citation topics: 1 Clinical & Life Sciences; 1.102 Stem Cell Research; 1.102.622 Pluripotency
Web Of Science research areas: Cell & Tissue Engineering; Cell Biology
ESI research areas: Molecular Biology & Genetics

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Details

Title: Structural phenotyping of stem cell-derived cardiomyocytes
Creators: Francesco Silvio Pasqualini - Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA
Sean Paul Sheehy - Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA
Ashutosh Agarwal - Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA
Yvonne Aratyn-Schaus - Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA
Kevin Kit Parker - Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, School of Engineering and Applied Sciences, Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA. Electronic address: kkparker@seas.harvard.edu
Publication Details: Stem cell reports, Vol.4(3), pp.340-347
Publisher: United States
Grant note: U01 HL100408 / NHLBI NIH HHS UH3 TR000522 / NCATS NIH HHS
Academic Unit: College of Engineering; CoE - Biomedical Engineering
Language: English
Resource Type: Journal article
PMID: 25733020
Record Identifier: 991031562206102976