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Antigen retrieval using pH 3.5 glycine-HCl buffer or urea solution for immunohistochemical localization of Ki-67
Journal article   Peer reviewed

Antigen retrieval using pH 3.5 glycine-HCl buffer or urea solution for immunohistochemical localization of Ki-67

S R Shi, B Chaiwun, L Young, A Imam, R J Cote and C R Taylor
Biotechnic & histochemistry, Vol.69(4), pp.213-215
1994-07
PMID: 7918836

Abstract

Nuclear Proteins - analysis Lymph Nodes - chemistry Lymphoma, B-Cell - chemistry Citrates Tissue Fixation - methods Nuclear Proteins - radiation effects Lymph Nodes - cytology Formaldehyde Immunohistochemistry - methods Paraffin Embedding Neoplasm Proteins - radiation effects Urea Glycine - chemistry Solutions Ki-67 Antigen Lymphoma, B-Cell - pathology Hydrochloric Acid - chemistry Neoplasm Proteins - analysis Cell Nucleus - chemistry Buffers Microwaves Hydrogen-Ion Concentration
A new antibody (MIB-1) has been described, permitting the demonstration of Ki-67 proliferation antigen in paraffin sections. However, satisfactory results were obtained only after subjecting tissue sections to microwave based antigen retrieval in citrate buffer solution. Other buffer solutions produce equivalent or better results and also permit use of the original Ki-67 antibody, which hitherto has been considered ineffective for paraffin sections.

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Citation topics
1 Clinical & Life Sciences
1.239 Tissue Barriers
1.239.1578 Antigen Retrieval
Web Of Science research areas
Biotechnology & Applied Microbiology
Cell Biology
ESI research areas
Biology & Biochemistry

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#3 Good Health and Well-Being

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