Affinity-Based Enrichment of Circulating Tumor Cells

Zheng Ao; Richard J Cote; Ram H Datar

doi:10.1007/978-1-4939-3363-1_2

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Book chapter

Affinity-Based Enrichment of Circulating Tumor Cells

Zheng Ao, Richard J Cote and Ram H Datar

Circulating Tumor Cells, pp.17-28

Current Cancer Research, Springer New York

2016-02-04

DOI: https://doi.org/10.1007/978-1-4939-3363-1_2

Abstract

In vivo CTC culture

Circulating tumor cells

Affinity-based cell capture

Molecular characterization of CTC

Study of CTC in cancer has always been hampered by its rare existence in blood. In this chapter, we discuss one of first principles employed to capture CTC from cancer patients’ peripheral blood—the affinity-based enrichment of CTC. We briefly discuss the different technologies utilizing antibodies to capture CTC based on specific antigen expression. Then we address the downstream molecular and functional characterization of CTC by utilizing these technologies. We also discuss the limitations of affinity-based CTC enrichment.

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Details

Title: Affinity-Based Enrichment of Circulating Tumor Cells
Creators: Zheng Ao - Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami Miller School of Medicine, Miami, USA
Richard J Cote - Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami Miller School of Medicine, Miami, USA
Ram H Datar - Dr. John T. Macdonald Foundation Biomedical Nanotechnology Institute, University of Miami Miller School of Medicine, Miami, USA
Publication Details: Circulating Tumor Cells, pp.17-28
Series: Current Cancer Research
Publisher: Springer New York; New York, NY
ISSN: 2199-2592
Academic Unit: Miller School of Medicine; UMMG Department of Pathology
Language: English
Resource Type: Book chapter
Record Identifier: 991031600180102976