Diagnostic Procedures for Paraffin-Embedded Tissues Analysis in Pharmacogenomic Studies

Raffaele Palmirotta; Maria Laura De Marchis; Giorgia Ludovici; Patrizia Ferroni; Pasquale Abete; Fiorella Guadagni; David Della-Morte

doi:10.1007/978-1-4939-0956-8_4

Book chapter

Diagnostic Procedures for Paraffin-Embedded Tissues Analysis in Pharmacogenomic Studies

Raffaele Palmirotta, Maria Laura De Marchis, Giorgia Ludovici, Patrizia Ferroni, Pasquale Abete, Fiorella Guadagni and David Della-Morte

Pharmacogenomics in Drug Discovery and Development, pp.45-65

Methods in Molecular Biology, Springer New York

2014-07-03

DOI: https://doi.org/10.1007/978-1-4939-0956-8_4

PMID: 25150866

Abstract

Diagnostic technique

Paraffin tumoral tissue

Personalized therapy

Pharmacogenomic

In this book chapter we report our own experience of mutational analysis in selecting tailored anticancer treatments for solid tumors. Our Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy, routinely performs pharmacogenetic screenings for different genes such as K-ras, BRAF, KIT, PDGFRα, and EGFR on paraffin-embedded cancer sections. Therefore, the chapter describes the mutational analysis procedures on paraffin-embedded tumors aimed to predict individual response to anticancer therapy. These molecular diagnostic methodologies may help us in improving the translational impact of genetic information on clinical practice.

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Details

Title: Diagnostic Procedures for Paraffin-Embedded Tissues Analysis in Pharmacogenomic Studies
Creators: Raffaele Palmirotta - Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy
Maria Laura De Marchis - Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy
Giorgia Ludovici - Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy
Patrizia Ferroni - Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy
Pasquale Abete - Department of Clinical Medicine, Cardiovascular Science & Immunology, University of Naples “Federico II”, Naples, Italy
Fiorella Guadagni - Department of Advanced Biotechnologies and Bioimaging, IRCCS San Raffaele Pisana, Rome, Italy
David Della-Morte - Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy
Publication Details: Pharmacogenomics in Drug Discovery and Development, pp.45-65
Series: Methods in Molecular Biology
Publisher: Springer New York; New York, NY
ISSN: 1940-6029
Academic Unit: Miller School of Medicine; UMMG Department of Neurology
Language: English
Resource Type: Book chapter
PMID: 25150866
Record Identifier: 991031614994302976