Abstract
Lentiviral vectors are vehicles for gene delivery that were originally
derived from the human immunodeficiency virus type-1 (HIV-1) lentivirus.
These vectors are defective for replication, and thus considered
relatively safe, but are capable of stably integrating into the genomic
DNA of a broad range of dividing and nondividing mammalian cell types.
The ability to stably integrate at semi-random genomic positions make
lentiviral vectors a unique and ideal tool for studying stochastic
variation in gene expression. Here, we describe the experimental and
mathematical methods for using lentiviral vectors to study stochastic
noise in gene expression.
