Abstract
In our experimental research,1 hematoxylin/eosin (HE) and immunohistochemistry (heat shock protein 27 [HSP27] expression) were used for assessing pulmonary extracts. A semiquantitative score for degrees of leukostasis, congested capillaries, alveolar hemorrhage, inflammatory infiltrate within alveoli, and alveolar edema was employed for sequential microscopic fields selected by two blinded pathologists. Each successive field was graded in terms of severity of injury as follows: grade 0, normal tissue; grade 1, mild injury; grade 2, moderate injury; and grade 3, severe injury. The extent of lesions was graded as 0, absent; 1, 1–25% of the slide; 2, 26–50%; and 3, less than 50% of tissue affected. The mean score for successive fields corresponded to the individual score for each pulmonary extract. A value of p < 0.05 was considered significant.2
