Abstract
Photochromic compounds switch reversibly between states with distinct absorption properties in the visible region under the influence of optical stimulation. This chapter deals with switching of fluorescence with photochromic oxazines. It describes the design and synthesis of fluorophore‐oxazine dyads, and discusses fluorescence activation with fluorophore‐oxazine dyads. The unique combination of photochemical and photophysical properties associated with the fluorophore‐photochrome dyads entrapped within the micelles offers the opportunity to overcome diffraction and resolve structural features at the nanoscale. Our strategies for fluorescence activation with photochromic oxazines can eventually lead to the realization of an entire family of photoswitchable fluorophores for the visualization of biological samples with subdiffraction resolution.