Quantification of Free Floating and Matrix Bound Growth Factors Secreted by Dynamically-Stimulated Endothelial Cells

M. R Newman; N Kazi; C. M Dumont; G Dai; D. M Thompson

doi:10.1109/NEBEC.2013.130

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Conference proceeding

Quantification of Free Floating and Matrix Bound Growth Factors Secreted by Dynamically-Stimulated Endothelial Cells

M. R Newman, N Kazi, C. M Dumont, G Dai and D. M Thompson

2013 39th Annual Northeast Bioengineering Conference, pp.88-89

2013-04

DOI: https://doi.org/10.1109/NEBEC.2013.130

Abstract

basic fibroblast growth factor

Electronic countermeasures

endothelial cell

epithelial growth factor

extracellular matrix

Fibroblasts

Fluid flow

In vitro

laminar shear stress

Production

Stem cells

Stress

Laminar shear stress from fluid flow influences endothelial cell (EC) behavior by increasing the production of specific growth factors (GFs). Moreover, fluid flow affects extracellular matrix (ECM) binding kinetics. This study investigates the effects of fluid flow on ECs for 5 and 12 h. Epithelial GF (EGF) and basic fibroblast GF (bFGF), in both free floating and ECM bound form, were quantified using enzyme-linked immunosorbent assays (ELISAs). Both bound and free floating EGF and bFGF were found to be increased in cultures under flow compared to cultures under static conditions. These results give a comprehensive understanding of the dynamic EC microenvironment and will be used in future studies for manipulation of neural stem cell cultures.

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Details

Title: Quantification of Free Floating and Matrix Bound Growth Factors Secreted by Dynamically-Stimulated Endothelial Cells
Creators: M. R Newman - Rensselaer Polytechnic Institute
N Kazi - Rensselaer Polytechnic Institute
C. M Dumont - Rensselaer Polytechnic Institute
G Dai - Rensselaer Polytechnic Institute
D. M Thompson - Rensselaer Polytechnic Institute
Publication Details: 2013 39th Annual Northeast Bioengineering Conference, pp.88-89
Publisher: IEEE
Academic Unit: College of Engineering; CoE - Biomedical Engineering
Language: English
Resource Type: Conference proceeding
Record Identifier: 991031714131402976