Abstract
The goal of this thesis project is to develop a method to perform direct wavefront reconstruction of in vitro crystalline lenses from spot diagrams measured using a Laser Ray Tracing (LRT) system. The LRT system is combined with a commercial Spectral-Domain OCT system to deliver laser rays sequentially onto the in vitro crystalline lens. The spot position for each ray is recorded at different axial positions using a camera. A MATLAB program was developed to calculate the slope of the rays from the spot positions. The ray slopes were used to calculate the Zernike wavefront coefficients using a least square curve fitting algorithm. The method was tested on glass lenses, on cynomolgus monkey lenses, and on human lenses.