A method for removal of interference by ammonium sulphate in protein assay by Lowry's method

HPS Makkar; O Sharma; R Dawra; S Negi

Back

A method for removal of interference by ammonium sulphate in protein assay by Lowry's method

Journal article

Peer reviewed

A method for removal of interference by ammonium sulphate in protein assay by Lowry's method

HPS Makkar, O Sharma, R Dawra and S Negi

Indian journal of biochemistry & biophysics, Vol.20(5), pp.306-308

1983-01-01

PMID: 6676164

Abstract

ammonium sulfate

proteins

A method for the removal of ammonium sulphate interference in the assay of protein by Lowry's method has been presented. Interference was eliminated by the addition of sodium hydroxide equivalent to double the molar amount of ammonium sulphate present in the sample and heating in boiling water-bath for 15 min. The heating was obligatory to eliminate interference due to ammonium hydroxide formed from ammonium sulphate.

Metrics

1 Record Views

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Citation topics: 2 Chemistry; 2.166 Chromatography & Electrophoresis; 2.166.1212 Human Serum Albumin
Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics
ESI research areas: Biology & Biochemistry

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Details

Title: A method for removal of interference by ammonium sulphate in protein assay by Lowry's method
Creators: HPS Makkar
O Sharma
R Dawra
S Negi
Publication Details: Indian journal of biochemistry & biophysics, Vol.20(5), pp.306-308
Academic Unit: Miller School of Medicine; UMMG Department of Surgery; UMMG Dept of Surgery - Div of Surgical Oncology
Language: English
Resource Type: Journal article
PMID: 6676164
Record Identifier: 991031725693902976