Abstract
Glia are important for the function of touch receptors. Here, we present a protocol in Caenorhabditis elegans for calcium and chloride imaging in worm glia upon nose touch stimulation. We describe aspects of the procedure that are essential for data reproducibility, including worm immobilization, poking angle, and applied force. We then detail data processing and analysis of calcium and chloride transients in glia. This protocol can be used for other types of mechanical stimulations or for stimulation using odorants.
For complete details on the use and execution of this protocol, please refer to Fernandez-Abascal et al., 2022.
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•Fluorescence imaging in C. elegans upon nose touch stimulation•Gluing worms for in vivo recordings•Processing and analysis of calcium and chloride transients in glia•Useful for other types of mechanical stimulations or for stimulation using odorants
Publisher's note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Glia are important for the function of touch receptors. Here, we present a protocol in Caenorhabditis elegans for calcium and chloride imaging in worm glia upon nose touch stimulation. We describe aspects of the procedure that are essential for data reproducibility, including worm immobilization, poking angle, and applied force. We then detail data processing and analysis of calcium and chloride transients in glia. This protocol can be used for other types of mechanical stimulations or for stimulation using odorants.
