Introduction:
Linking markers together into large phase-blocks, and examining the interaction of different sequencing modalities, allows complex genomic and epigenomic states to be integrated to generate true multiomic haplotypes at the chromosomal level.
Methods:
Fourteen multiple myeloma patient-derived xenografts were used to generate multiomic data including short- (Illumina) and long-read (PacBio) whole genome sequencing (WGS) to identify single nucleotide variations (SNVs), copy number (CN) abnormalities, structural variation (SV), and DNA methylation, as well as expression, chromatin states (Cut&Tag-IT), and Micro-C/LinkPrep (Dovetail Genomics) to identify 3D chromatin architecture. Chromosome-scale haplotypes of SNVs, SVs, CNVs, DNA methylation, expression, and chromatin marks were generated.
Results:
For the first time, complete haplotype-resolved assemblies at the chromosomal level have been generated in multiple myeloma. A 100x coverage genome Micro-C/LinkPrep libraries we were able to phase the long and short arms of each chromosome, resulting in an average 94.3% haplotype phasing per autosome - thereby generating chromosome length haplotypes up to 241.9 Mb. Haplotype-specific heatmaps were generated allowing us to examine the interaction of complex SVs across chromosomes. In one sample, we identified a primary t(11;14) and additional SV events linked to the t(11;14) including a t(3;14), t(11;17), and t(3;17) which created a cyclical pattern. We found that 70-83.5% of reads support specific combinations of haplotype interactions, and that all four SV events were linked and involved the same haplotypes on each chromosome. The pattern of interactions combined with breakpoint analysis in this case indicated a four-way complex reciprocal translocation between chromosomes 3,11,14 and 17. Integration of epigenetic data in this complex SV showed DNA hyper-methylation 17 kb upstream of CCND1 next to the t(11;14) breakpoint as well as increased H3K27ac marks on the same haplotype, indicating spreading of the activating broad domain from the IGH super-enhancer on chromosome 14. Equally, the hypomethylated DNA marks at the IGH promoter are spread to chromosome 3, via the t(3;14), resulting in over-expression of the proto-oncogene SKIL. The same is true for the t(3;17), which shows hypomethylation on both sides of the breakpoint, compared to the non-translocated allele.
Conclusion:
We have generated the first chromosome scale haplotype-resolved genomes in multiple myeloma and integrated them with epigenetic states to identify interactions across chromosomes and resolve complex SVs as well as their epigenomic consequences to understand the intricate nature of how the genome is organized.
Citation Format:
Nathan J. Becker, Enze Liu, J. Zachary Sanborn, Attaya Suvannasankha, Kelvin Lee, Dickran Kazandjian, Benjamin Diamond, Abhishek Pandey, Rafat Abonour, Ola Landgren, Elizabeth M. Munding, Aneta Mikulasova, Brian A. Walker. Chromosome-level phasing to resolve haplotypes using multiomic data in multiple myeloma reveals complex distal interactions between chromosomes that impacts epigenetic states and gene expression [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 5940.
- Abstract 5940: Chromosome-level phasing to resolve haplotypes using multiomic data in multiple myeloma reveals complex distal interactions between chromosomes that impacts epigenetic states and gene expression
- Nathan J. Becker - University of MiamiJ. Zachary Sanborn - Dovetail Genomics (United States)Attaya Suvannasankha - University of IndianapolisKelvin Lee - Indiana University – Purdue University IndianapolisDickran Kazandjian - University of MiamiBenjamin Diamond - University of MiamiAbhishek Pandey - University of MiamiRafat Abonour - University of MiamiOla Landgren - University of MiamiElizabeth M. Munding - Dovetail Genomics (United States)Aneta Mikulasova - University of EdinburghBrian A. Walker - University of MiamiEnze Liu - University of Miami, Miller School of Medicine
- Cancer research (Chicago, Ill.), Vol.86(7_Supplement), 5940
- AMER ASSOC CANCER RESEARCH
- 1
- Miller School of Medicine; UMMG Dept of Medicine - Hematology/Oncology; UMMG Department of Medicine - Myeloma
- English
- Journal article
- 991033052193302976