Age-related differences in cigarette smoke extract-induced H 2O 2 production by lung endothelial cells

C.A. Downs; D.W. Montgomery; C.J. Merkle

doi:10.1016/j.mvr.2011.09.013

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Journal article

Age-related differences in cigarette smoke extract-induced H 2O 2 production by lung endothelial cells

C.A. Downs, D.W. Montgomery and C.J. Merkle

Microvascular Research, Vol.82(3), pp.311-317

2011

DOI: https://doi.org/10.1016/j.mvr.2011.09.013

Abstract

cigarette smoke

hydrogen peroxide

hydroxyl radical

nitric oxide

peroxynitrite

reactive oxygen metabolite

age

animal cell

animal cell culture

apolipoprotein E gene

article

coculture

controlled study

cytoglobin gene

excision repair cross complementing rodent repair deficiency gene

Fischer 344 rat

gene expression profiling

lung endothelium

male

microvascular endothelial cell

newborn

nonhuman

prion protein gene

priority journal

rat

reduced nicotinamide adenine dinucleotide (phosphate) dehydrogenase (quinone) 1 gene

regulator gene

solute carrier family 38 member 1 gene

tropomodulin 1 gene

zinc finger MYND type containing 17 gene

Age Factors

Animals

Cells, Cultured

Coculture Techniques

Dose-Response Relationship, Drug

Endothelial Cells

Gene Expression Regulation

Lung

Pneumocytes

Rats

Rats, Inbred F344

Smoke

Smoking

Rattus

Oxidative Stress

Aging

Antioxidants

Cigarette smoke causes oxidative stress in the lung resulting in injury and disease. The purpose of this study was to determine if there were age-related differences in cigarette smoke extract (CSE)-induced production of reactive species in single and co-cultures of alveolar epithelial type I (AT I) cells and microvascular endothelial cells harvested from the lungs (MVECLs) of neonatal, young and old male Fischer 344 rats. Cultures of AT I cells and MVECLs grown separately (single culture) and together (co-culture) were exposed to CSE (1, 10, 50, 100%). Cultures were assayed for the production of intracellular reactive oxygen species (ROS), hydroxyl radical (OH), peroxynitrite (ONOO -), nitric oxide (NO) and extracellular hydrogen peroxide (H 2O 2). Single and co-cultures of AT I cells and MVECLs from all three ages produced minimal intracellular ROS in response to CSE. All ages of MVECLs produced H 2O 2 in response to CSE, but young MVECLs produced significantly less H 2O 2 compared to neonatal and old MVECLs. Interestingly, when grown as a co-culture with age-matched AT I cells, neonatal and old MVECLs demonstrated ~50% reduction in H 2O 2 production in response to CSE. However, H 2O 2 production in young MVECLs grown as a co-culture with young AT I cells did not change with CSE exposure. To begin investigating for a potential mechanism to explain the reduction in H 2O 2 production in the co-cultures, we evaluated single and co-cultures for extracellular total antioxidant capacity. We also performed gene expression profiling specific to oxidant and anti-oxidant pathways. The total antioxidant capacity of the AT I cell supernatant was ~5 times greater than that of the MVECLs, and when grown as a co-culture and exposed to CSE (≥10%), the total antioxidant capacity of the supernatant was reduced by ~50%. There were no age-related differences in total antioxidant capacity of the cell supernatants. Gene expression profiling found eight genes to be significantly up-regulated or down-regulated. This is the first study to describe age-related differences in MVECLs exposed to CSE. © 2011 Elsevier Inc.

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.65 Allergy; 1.65.192 COPD
Web Of Science research areas: Peripheral Vascular Disease
ESI research areas: Clinical Medicine

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Title: Age-related differences in cigarette smoke extract-induced H 2O 2 production by lung endothelial cells
Creators: C.A. Downs - University of Arizona
D.W. Montgomery - University of Arizona
C.J. Merkle - University of Arizona
Publication Details: Microvascular Research, Vol.82(3), pp.311-317
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Academic Unit: School of Nursing - Research; School of Nursing & Health Studies
Language: English
Resource Type: Journal article
Record Identifier: 991031658303802976