Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry

Emily J Neag; Vanessa Collao; Sanjoy K Bhattacharya

doi:10.1007/978-1-0716-2966-6_13

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Journal article

Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry

Emily J Neag, Vanessa Collao and Sanjoy K Bhattacharya

Methods in molecular biology (Clifton, N.J.), Vol.2625, pp.141-148

2023

DOI: https://doi.org/10.1007/978-1-0716-2966-6_13

PMID: 36653640

Appears in Miller School of Medicine - Latest Publications

Abstract

Cholesterol

Cholesterol Esters

Gas Chromatography-Mass Spectrometry - methods

Phytosterols

Sterols - analysis

An optimized Bligh and Dyer protocol and subsequent derivatization is described in this chapter for the extraction of free cholesterol and cholesterol esters from tissue samples. Quantification analysis of lipid species is then described utilizing gas chromatography-mass spectrometry, the ideal method for analysis of volatile organic compounds and extraction of sterols.

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Title: Analysis of Cholesterol Lipids Using Gas Chromatography Mass Spectrometry
Creators: Emily J Neag - University of Miami
Vanessa Collao - University of Miami
Sanjoy K Bhattacharya - Miami Integrative Metabolomics Research Center, Miami, FL, USA. neagemil@msu.edu
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.2625, pp.141-148
ISBN: 9781071629666; 1071629662
Academic Unit: Bascom Palmer Eye Institute; UMMG Department of Ophthalmology; Miller School of Medicine
Language: English
Resource Type: Journal article
PMID: 36653640
Record Identifier: 991032796181602976