Abstract
The antigen retrieval (AR) technique is a novel, simple, and reliable approach to routine immunohistochemistry (IHC) developed in 1991. It has increasingly been used in pathology and analytical morphology, with a wide fruitful field opened for both clinical and research projects. Hundreds of articles concerning AR-IHC have been published in a variety of journals all over the world. As a recently developed technique, the wide use of AR has raised numerous questions for further study. This review article focuses on several key points of the method and further developments of the AR technique that are different from some review articles published recently. (The J Histotechnol 20:145, 1997)