Abstract
The magnitude of the problems of drug abuse and Neuro-AIDS warrants the development of novel approaches for testing
hypotheses in diagnosis and treatment ranging from cell culture models to developing databases. In this study, cultured
neurons were treated with/without HIV-TAT, ENV, or cocaine in a 2x2x2 expression study design. RNA was purified, labeled,
and expression data were produced and analyzed using ANOVA. Thus, we identified 35 genes that were significantly expressed
across treatment conditions. A diagram is presented showing examples of molecular relationships involving a significantly
expressed gene in the current study (SOX2). Also, we use this information to discuss examples of gene expression interactions
as a means to portray significance and complexity of gene expression studies in Drug Abuse and Neuro-AIDS. Furthermore, we
discuss here that critical interactions remain undetected, which may be unravelled by developing robust database systems
containing large datasets and gleaned information from collaborating scientists . Hence, we are developing a public domain
database we named
The Agora
database , that will served as a shared infrastructure to query, deposit, and review information
related to drug abuse and dementias including Neuro-AIDS. A workflow of this database is also outlined in this paper.
