Bioluminescent stem-loop probes for highly sensitive nucleic acid detection

Eric A Hunt; Sapna K Deo

doi:10.1039/c1cc13495g

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Journal article

Bioluminescent stem-loop probes for highly sensitive nucleic acid detection

Eric A Hunt and Sapna K Deo

Chemical communications (Cambridge, England), Vol.47(33), pp.9393-9395

2011-09-07

DOI: https://doi.org/10.1039/c1cc13495g

PMID: 21769413

Abstract

Protein Structure, Tertiary

Fluorescent Dyes - chemistry

Nucleic Acids - analysis

Luminescent Measurements - methods

Luciferases, Renilla - chemistry

Here, we report the first bioluminescent stem-loop probe, which is 50 times more sensitive and able to achieve a LOD 25 times lower than fluorescent stem-loop probes. Chemical generation of a signal from Renilla luciferase reduces background noise for improved quantitative utility in nucleic acid biomarker detection.

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Citation topics: 2 Chemistry; 2.145 Biosensors; 2.145.243 Aptamer
Web Of Science research areas: Chemistry, Multidisciplinary
ESI research areas: Chemistry

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Details

Title: Bioluminescent stem-loop probes for highly sensitive nucleic acid detection
Creators: Eric A Hunt - Department of Biochemistry & Molecular Biology, University of Miami Miller School of Medicine, Miami, FL 33136, USA
Sapna K Deo
Publication Details: Chemical communications (Cambridge, England), Vol.47(33), pp.9393-9395
Publisher: England
Academic Unit: Miller School of Medicine; Biochemistry & Molecular Biology Research
Language: English
Resource Type: Journal article
PMID: 21769413
Record Identifier: 991031560003902976