Cloning and Functional Characterization of the Early-Lymphocyte-Specific Pb99 Gene

Barry P Sleckman; Wasif N Khan; Wanping Xu; Craig H Bassing; Barbara A Malynn; Neal G Copeland; Christiana G Bardon; Timo M Breit; Laurie Davidson; Eugene M Oltz; Nancy A Jenkins; Jeffrey E Berman; Frederick W Alt

doi:10.1128/MCB.20.12.4405-4410.2000

Back

Cloning and Functional Characterization of the Early-Lymphocyte-Specific Pb99 Gene

Journal article

Open access

Peer reviewed

Cloning and Functional Characterization of the Early-Lymphocyte-Specific Pb99 Gene

Barry P Sleckman, Wasif N Khan, Wanping Xu, Craig H Bassing, Barbara A Malynn, Neal G Copeland, Christiana G Bardon, Timo M Breit, Laurie Davidson, Eugene M Oltz, …

Molecular and cellular biology, Vol.20(12), pp.4405-4410

2000-06

DOI: https://doi.org/10.1128/MCB.20.12.4405-4410.2000

PMCID: PMC85807

PMID: 10825203

Abstract

Mammalian Genetic Models with Minimal or Complex Phenotypes

The Pb99 gene is specifically expressed in pre-B cells and thymocytes and not in mature B and T cells or nonlymphoid tissues, implying that it may function in early lymphoid development. We have previously described the cloning of an incomplete cDNA for Pb99 . Here we report the isolation of full-length cDNAs and genomic clones for the murine Pb99 gene and the mapping of its location to mouse chromosome 8. Sequence analyses of different Pb99 cDNA clones suggest that there may be at least three forms of the Pb99 protein generated by differential processing of the Pb99 transcript. The cDNA with the longest open reading frame encodes a putative protein that has seven hydrophobic domains similar to those of seven membrane-spanning proteins, such as the classical G protein-coupled receptors. To directly address the role of the Pb99 protein in lymphoid development, Pb99-deficient mice were generated by gene targeting, and lymphocyte development in these mice was analyzed.

Files and links (1)

url

https://doi.org/10.1128/MCB.20.12.4405-4410.2000View

Published (Version of record) Open

Metrics

16 Record Views

12 Times Cited - Web of Science

See more details

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Industry collaboration; Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.6 Immunology; 1.6.452 Somatic Hypermutation
Web Of Science research areas: Biochemistry & Molecular Biology; Cell Biology
ESI research areas: Molecular Biology & Genetics

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Details

Title: Cloning and Functional Characterization of the Early-Lymphocyte-Specific Pb99 Gene
Creators: Barry P Sleckman - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Wasif N Khan - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Wanping Xu - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Craig H Bassing - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Barbara A Malynn - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Neal G Copeland - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Christiana G Bardon - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Timo M Breit - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Laurie Davidson - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Eugene M Oltz - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Nancy A Jenkins - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Jeffrey E Berman - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Frederick W Alt - Howard Hughes Medical Institute, Children's Hospital, and Department of Genetics, Harvard Medical School, and The Center for Blood Research, Boston, Massachusetts 02115
Publication Details: Molecular and cellular biology, Vol.20(12), pp.4405-4410
Publisher: American Society for Microbiology
Academic Unit: Miller School of Medicine; Microbiology and Immunology Research
Language: English
Resource Type: Journal article
PMID: 10825203
PMCID: PMC85807
Record Identifier: 991031562432002976