Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Kate E Keller; Sanjoy K Bhattacharya; Theresa Borrás; Thomas M Brunner; Sunee Chansangpetch; Abbott F Clark; W. Michael Dismuke; Yiqin Du; Michael H Elliott; C. Ross Ethier; Jennifer A Faralli; Thomas F Freddo; Rudolf Fuchshofer; Michael Giovingo; Haiyan Gong; Pedro Gonzalez; Alex Huang; Murray A Johnstone; Paul L Kaufman; Mary J Kelley; Paul A Knepper; Casey C Kopczynski; John G Kuchtey; Rachel W Kuchtey; Markus H Kuehn; Raquel L Lieberman; Shan C Lin; Paloma Liton; Yutao Liu; Elke Lütjen-Drecoll; Weiming Mao; Marisse Masis-Solano; Fiona McDonnell; Colleen M McDowell; Darryl R Overby; Padmanabhan P Pattabiraman; Vijay K Raghunathan; P. Vasanth Rao; Douglas J Rhee; Uttio Roy Chowdhury; Paul Russell; John R Samples; Donald Schwartz; Evan B Stubbs; Ernst R Tamm; James C Tan; Carol B Toris; Karen Y Torrejon; Janice A Vranka; Mary K Wirtz; Thomas Yorio; Jie Zhang; Gulab S Zode; Michael P Fautsch; Donna M Peters; Ted S Acott; W. Daniel Stamer

doi:10.1016/j.exer.2018.03.001

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Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Journal article

Open access

Peer reviewed

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Kate E Keller, Sanjoy K Bhattacharya, Theresa Borrás, Thomas M Brunner, Sunee Chansangpetch, Abbott F Clark, W. Michael Dismuke, Yiqin Du, Michael H Elliott, C. Ross Ethier, …

Experimental eye research, Vol.171, pp.164-173

2018-06

DOI: https://doi.org/10.1016/j.exer.2018.03.001

PMCID: PMC6042513

PMID: 29526795

Abstract

Glaucoma

Conventional outflow

Aqueous humor dynamics

Ocular hypertension

Intraocular pressure

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings. •Comprehensive review of techniques to culture TM cells are presented.•Helpful tips for culturing TM cells are described.•First set of consensus standards for TM cell research is defined.

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.36 Ophthalmology; 1.36.226 Glaucoma
Web Of Science research areas: Ophthalmology
ESI research areas: Clinical Medicine

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Title: Consensus recommendations for trabecular meshwork cell isolation, characterization and culture
Creators: Kate E Keller - Oregon Health and Science University, United States
Sanjoy K Bhattacharya - University of Miami, United States
Theresa Borrás - University of North Carolina-Chapel Hill, United States
Thomas M Brunner - Glaucoma Research Foundation, United States
Sunee Chansangpetch - University of California, San Francisco, United States
Abbott F Clark - University of North Texas Health Sciences Center, United States
W. Michael Dismuke - Aerie Pharmaceuticals, United States
Yiqin Du - University of Pittsburgh, United States
Michael H Elliott - University of Oklahoma, United States
C. Ross Ethier - Georgia Institute of Technology, United States
Jennifer A Faralli - University of Wisconsin, United States
Thomas F Freddo - Massachusetts College of Pharmacy and Health Sciences, United States
Rudolf Fuchshofer - University of Regensburg, Germany
Michael Giovingo - John H. Stroger, Jr. Hospital of Cook County, United States
Haiyan Gong - Boston University, United States
Pedro Gonzalez - Duke University, United States
Alex Huang - University of California, Los Angeles, United States
Murray A Johnstone - University of Washington, United States
Paul L Kaufman - University of Wisconsin, United States
Mary J Kelley - Oregon Health and Science University, United States
Paul A Knepper - University of Illinois at Chicago, United States
Casey C Kopczynski - Aerie Pharmaceuticals, United States
John G Kuchtey - Vanderbilt University, United States
Rachel W Kuchtey - Vanderbilt University, United States
Markus H Kuehn - University of Iowa, United States
Raquel L Lieberman - Georgia Institute of Technology, United States
Shan C Lin - University of California, San Francisco, United States
Paloma Liton - Duke University, United States
Yutao Liu - Augusta University, United States
Elke Lütjen-Drecoll - University of Erlangen-Nuremberg, Germany
Weiming Mao - University of North Texas Health Sciences Center, United States
Marisse Masis-Solano - University of California, San Francisco, United States
Fiona McDonnell - Duke University, United States
Colleen M McDowell - University of North Texas Health Sciences Center, United States
Darryl R Overby - Imperial College, London, United Kingdom
Padmanabhan P Pattabiraman - Case Western Reserve University, United States
Vijay K Raghunathan - University of Houston, United States
P. Vasanth Rao - Duke University, United States
Douglas J Rhee - Case Western Reserve University, United States
Uttio Roy Chowdhury - Mayo Clinic, United States
Paul Russell - University of California, Davis, United States
John R Samples - Western Glaucoma Foundation, United States
Donald Schwartz - EyeSonix, United States
Evan B Stubbs - Loyola University, Chicago, United States
Ernst R Tamm - University of Regensburg, Germany
James C Tan - University of Southern California, United States
Carol B Toris - Case Western Reserve University, United States
Karen Y Torrejon - Glauconix, United States
Janice A Vranka - Oregon Health and Science University, United States
Mary K Wirtz - Oregon Health and Science University, United States
Thomas Yorio - University of North Texas Health Sciences Center, United States
Jie Zhang - University of California, Los Angeles, United States
Gulab S Zode - University of North Texas Health Sciences Center, United States
Michael P Fautsch - Department of Ophthalmology, Mayo Clinic, 200 1st St SW, Rochester, MN 55905, United States
Donna M Peters - Department of Pathology & Laboratory Medicine, University of Wisconsin, 1300 University Ave, Madison, WI 53706, United States
Ted S Acott - Department of Ophthalmology, Department of Biochemistry & Molecular Biology, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, United States
W. Daniel Stamer - Department of Ophthalmology, Duke University, DUMC 3802, Durham, NC 27705, United States
Publication Details: Experimental eye research, Vol.171, pp.164-173
Publisher: Elsevier Ltd
Academic Unit: Miller School of Medicine; UMMG Department of Ophthalmology
Language: English
Resource Type: Journal article
PMID: 29526795
PMCID: PMC6042513
Record Identifier: 991031596994102976