Abstract
We describe the construction of a multi‐immobilized restriction endonuclease module (Multi RE module). We demonstrate that the applied mechanical stress enables modulation of enzyme activity and modulation of recognition site selectivity (in oligonucleotides of ∼200 bp) of immobilized restriction endonucleases. The central module which is consisted of different strips of immobilized restriction endonucleases allows limited digestion of a large DNA sample in a controlled manner as a function of applied mechanical stress on strips. The stress–activity relationship and the effect of repeated cycles of stress and relaxation on the immobilized strips are presented here. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 65: 233–239, 1999.
