CpG Methylation as a Tool to Characterize Cell-Free Kaposi Sarcoma Herpesvirus DNA

Meir Shamay; Nicholas Hand; M. Victor Lemas; Henry B Koon; Susan E Krown; John Wrangle; Prashant Desai; Juan Carlos Ramos; Richard F Ambinder

doi:10.1093/infdis/jis032

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CpG Methylation as a Tool to Characterize Cell-Free Kaposi Sarcoma Herpesvirus DNA

Journal article

Open access

Peer reviewed

CpG Methylation as a Tool to Characterize Cell-Free Kaposi Sarcoma Herpesvirus DNA

Meir Shamay, Nicholas Hand, M. Victor Lemas, Henry B Koon, Susan E Krown, John Wrangle, Prashant Desai, Juan Carlos Ramos and Richard F Ambinder

The Journal of infectious diseases, Vol.205(7), pp.1095-1099

2012-04-01

DOI: https://doi.org/10.1093/infdis/jis032

PMCID: PMC3295606

PMID: 22357696

Abstract

Major and Brief Reports

(See the editorial commentary by Stebbing and Bower, on pages 1032–4 .) We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.130 Lymphomas; 1.130.1220 Kaposi
Web Of Science research areas: Immunology; Infectious Diseases; Microbiology
ESI research areas: Immunology

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Details

Title: CpG Methylation as a Tool to Characterize Cell-Free Kaposi Sarcoma Herpesvirus DNA
Creators: Meir Shamay - Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland
Nicholas Hand - Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland
M. Victor Lemas - Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland
Henry B Koon - Seidman Cancer Center, Cleveland, Ohio
Susan E Krown - Memorial Sloan-Kettering Cancer Center, New York, New York
John Wrangle - Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland
Prashant Desai - Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland
Juan Carlos Ramos - Division of Hematology-Oncology, Sylvester Comprehensive Cancer Center, Miami, Florida
Richard F Ambinder - Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland
Publication Details: The Journal of infectious diseases, Vol.205(7), pp.1095-1099
Publisher: Oxford University Press
Academic Unit: Miller School of Medicine; UMMG Dept of Medicine - Hematology/Oncology
Language: English
Resource Type: Journal article
PMID: 22357696
PMCID: PMC3295606
Record Identifier: 991031576886602976