Abstract
Unavailability of fusion tags that possess both affinity and visualization properties is a hurdle for biomolecular research. Typically, either a choice is made between an affinity tag and a reporter tag or both are employed in tandem if a fusion can be made at both termini of the target biomolecule. In this work, we have developed a site-specific genetic fusion approach employing DsRed-Monomer, a red fluorescent protein, that provides for both affinity and reporter functionality in a single tag. As a proof-of-concept, two fusion proteins, bradykinin-DsRed-Monomer and calmodulin-DsRed-Monomer, were prepared for the study. These fusion proteins were purified using a copper-immobilized column based on the inherent copper-binding property of DsRed-Monomer. Spectroscopic characterization of fusion proteins and comparison with native DsRed-Monomer showed no effect of fusion on the properties of DsRed-Monomer. Further, bradykinin-DsRed-Monomer was employed in the development of a competitive fluorescence immunoassay for the peptide bradykinin. Calmodulin-DsRed-Monomer was used to detect binding of the calmodulin ligand chlorpromazine, based on a change in the fluorescence of DsRed-Monomer upon binding of chlorpromazine to calmodulin. The studies performed demonstrate the application of DsRed-Monomer as a dual function tag indicating the potential usefulness of DsRed-monomer in proteomics and biomolecular research.
