Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)

Daniela Frasca; Paola Barattini; Donatella Tirindelli; Luisa Guidi; Carlo Bartoloni; Andrea Errani; Mario Costanzo; Augusto Tricerri; Luca Pierelli; Gino Doria

doi:10.1016/S0531-5565(99)00026-1

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Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)

Journal article

Peer reviewed

Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)

Daniela Frasca, Paola Barattini, Donatella Tirindelli, Luisa Guidi, Carlo Bartoloni, Andrea Errani, Mario Costanzo, Augusto Tricerri, Luca Pierelli and Gino Doria

Experimental gerontology, Vol.34(5), pp.645-658

1999

DOI: https://doi.org/10.1016/S0531-5565(99)00026-1

PMID: 10530790

Abstract

Ku protein

Aging

DNA repair

DNA binding of the ku protein was investigated in peripheral blood mononuclear cells (PBMC) from 24 subjects of different ages (20–89 years old) displaying age-related changes in DNA repair, mitotic responsiveness, and cytokine production. Ku is an heterodimeric protein composed of two subunits of 70 and 80 kDa, which is involved in the earliest steps of DNA damage recognition. DNA binding of ku 70/80 was found unchanged in normal PBMC from aging subjects but progressively declined in x-ray-irradiated PBMC from young to adult, and elderly subjects. This finding was concomitant with the age-related fall of DNA repair in the whole population.

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.152 Molecular & Cell Biology - DNA Damage; 1.152.257 DSBs
Web Of Science research areas: Geriatrics & Gerontology
ESI research areas: Clinical Medicine

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Details

Title: Effect of age on DNA binding of the ku protein in irradiated human peripheral blood mononuclear cells (PBMC)
Creators: Daniela Frasca - Laboratory of Immunology, AMB-PRO-TOSS, ENEA CR Casaccia, Via Anguillarese 301, 00060 S. Maria di Galeria, Rome, Italy
Paola Barattini - Laboratory of Immunology, AMB-PRO-TOSS, ENEA CR Casaccia, Via Anguillarese 301, 00060 S. Maria di Galeria, Rome, Italy
Donatella Tirindelli - Laboratory of Immunology, AMB-PRO-TOSS, ENEA CR Casaccia, Via Anguillarese 301, 00060 S. Maria di Galeria, Rome, Italy
Luisa Guidi - Institute of Internal Medicine, Catholic University of Rome, Rome, Italy
Carlo Bartoloni - Institute of Internal Medicine, Catholic University of Rome, Rome, Italy
Andrea Errani - Institute of Internal Medicine, Catholic University of Rome, Rome, Italy
Mario Costanzo - Institute of Internal Medicine, Catholic University of Rome, Rome, Italy
Augusto Tricerri - Institute of Internal Medicine, Catholic University of Rome, Rome, Italy
Luca Pierelli - Hematology Unit, Catholic University of Rome, Rome, Italy
Gino Doria - Department of Biology, University of Rome Tor Vergata, Italy
Publication Details: Experimental gerontology, Vol.34(5), pp.645-658
Publisher: Elsevier Inc
Academic Unit: Miller School of Medicine; Microbiology and Immunology Research
Language: English
Resource Type: Journal article
PMID: 10530790
Record Identifier: 991031560655302976