Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

N F Tsinoremas; A K Kutach; C A Strayer; S S Golden

doi:10.1128/jb.176.21.6764-6768.1994

Back

Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

Journal article

Open access

Peer reviewed

Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination

N F Tsinoremas, A K Kutach, C A Strayer and S S Golden

Journal of bacteriology, Vol.176(21), pp.6764-6768

1994-11

DOI: https://doi.org/10.1128/jb.176.21.6764-6768.1994

PMCID: PMC197036

PMID: 7961432

Abstract

We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.

Files and links (1)

url

https://doi.org/10.1128/jb.176.21.6764-6768.1994View

Published (Version of record) Open

Metrics

8 Record Views

57 Times Cited - Web of Science

See more details

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Citation topics: 3 Agriculture, Environment & Ecology; 3.171 Photoproductivity; 3.171.477 Microalgae
Web Of Science research areas: Microbiology
ESI research areas: Microbiology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Details

Title: Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination
Creators: N F Tsinoremas - Department of Biology, Texas A&M University, College Station 77843-3258
A K Kutach - Department of Biology, Texas A&M University, College Station 77843-3258
C A Strayer - Department of Biology, Texas A&M University, College Station 77843-3258
S S Golden - Department of Biology, Texas A&M University, College Station 77843-3258
Publication Details: Journal of bacteriology, Vol.176(21), pp.6764-6768
Academic Unit: Institute for Data Science and Computing; Biochemistry & Molecular Biology Research; Institute for Data Science and Computing Administration
Language: English
Resource Type: Journal article
PMID: 7961432
PMCID: PMC197036
Record Identifier: 991031613359102976