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Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate
Journal article   Peer reviewed

Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate

P S Cassidy and W G Kerrick
Biochimica et biophysica acta, Vol.565(1), pp.209-213
1979-11-22
PMID: 508762

Abstract

Phosphorylation Adenosine Diphosphate Thionucleotides - chemical synthesis Adenosine Triphosphate - chemical synthesis Glyceraldehyde 3-Phosphate Sulfur Radioisotopes Phosphotransferases Adenosine Triphosphate - analogs & derivatives Methods
The very useful radiolabeled ATP analog, adenosine 5-O-(3-[35S] thiotriphosphate) or [35S] ATPgammaS, has been prepared by a technique based on the thiophosphorylation of ADP that results in much higher yields of [35S] ATPgammaS than does a thiophosphate exchange method [1].

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