Extracellular Vesicles with Exosome-like Features Transfer TLRs between Dendritic Cells

Yue Zhang; Jingjing Meng; Li Zhang; Sundaram Ramkrishnan; Sabita Roy

doi:10.4049/immunohorizons.1900016

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Extracellular Vesicles with Exosome-like Features Transfer TLRs between Dendritic Cells

Journal article

Peer reviewed

Extracellular Vesicles with Exosome-like Features Transfer TLRs between Dendritic Cells

Yue Zhang, Jingjing Meng, Li Zhang, Sundaram Ramkrishnan and Sabita Roy

ImmunoHorizons, Vol.3(6), pp.186-193

2019-06-04

DOI: https://doi.org/10.4049/immunohorizons.1900016

PMID: 31356164

Abstract

Exosomes - metabolism

Signal Transduction

Dendritic Cells - immunology

Extracellular Vesicles - pathology

Mice, Inbred C57BL

Cells, Cultured

Toll-Like Receptor 4 - genetics

Exosomes - pathology

NF-kappa B - metabolism

Inflammation - immunology

Lipopolysaccharides - immunology

Toll-Like Receptor 4 - metabolism

Extracellular Vesicles - metabolism

Mice, Knockout

Animals

Cell Differentiation

Mice

Accumulating evidence shows that extracellular vesicles (EVs) secreted by immune cells play an important role in intercellular communication. In the current report, we show that EVs released from wild-type bone marrow-derived dendritic cells (BMDCs) transfer TLRs to TLR4-knockout (TLR4KO) BMDCs and increase cellular responsiveness to LPS in recipient cells. The transferred EVs have exosomal characteristics and induce the activation of NF-κB signaling pathways in recipient cells. We further show that BMDC-derived EVs can promote LPS-induced inflammation in TLR4KO mice in vivo. These results indicate that functional TLR4 can be transferred from wild-type to TLR4KO BMDCs through exosome-like EVs.

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Title: Extracellular Vesicles with Exosome-like Features Transfer TLRs between Dendritic Cells
Creators: Yue Zhang - Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL 33101; and
Jingjing Meng - Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL 33101; and
Li Zhang - Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455
Sundaram Ramkrishnan - Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL 33101; and
Sabita Roy - Department of Surgery, Miller School of Medicine, University of Miami, Miami, FL 33101; and sabita.roy@miami.edu
Publication Details: ImmunoHorizons, Vol.3(6), pp.186-193
Grant note: R01 DK117576 / NIDDK NIH HHS R01 DA050542 / NIDA NIH HHS R01 DA044582 / NIDA NIH HHS R01 DA034582 / NIDA NIH HHS R01 DA047089 / NIDA NIH HHS R01 DA037843 / NIDA NIH HHS R01 DA043252 / NIDA NIH HHS
Academic Unit: Miller School of Medicine; UMMG Department of Surgery; UMMG Dept of Surgery - Div of Surgical Oncology
Language: English
Resource Type: Journal article
PMID: 31356164
Record Identifier: 991031597728102976