Abstract
To develop, test and validate functional slit lamp biomicroscopy (FSLB) for generating non-invasive bulbar conjunctival microvascular perfusion maps (nMPMs) and assessing morphometry and hemodynamics.
FSLB was adapted from a traditional slit-lamp microscope by attaching a digital camera to image the bulbar conjunctiva to create nMPMs and measure venular blood flow hemodynamics. High definition images with a large field of view were obtained on the temporal bulbar conjunctiva for creating nMPMs. A high imaging rate of 60frames per second and an ~210× high magnification were achieved using the camera inherited high speed setting and Movie Crop Function, for imaging hemodynamics. Custom software was developed to segment bulbar conjunctival nMPMs for further fractal analysis and quantitatively measure blood vessel diameter, blood flow velocity and flow rate. Six human subjects were imaged before and after 6h of wearing contact lenses. Monofractal and multifractal analyses were performed to quantify fractality of the nMPMs.
The mean bulbar conjunctival vessel diameter was 18.8±2.7μm at baseline and increased to 19.6±2.4μm after 6h of lens wear (P=0.020). The blood flow velocity was increased from 0.60±0.12mm/s to 0.88±0.21mm/s (P=0.001). The blood flow rate was also increased from 129.8±59.9pl/s to 207.2±81.3pl/s (P=0.001). Bulbar conjunctival nMPMs showed the intricate details of the bulbar conjunctival microvascular network. At baseline, fractal dimension was 1.63±0.05 and 1.71±0.03 analyzed by monofractal and multifractal analyses, respectively. Significant increases in fractal dimensions were found after 6h of lens wear (P<0.05).
Microvascular network's fractality, morphometry and hemodynamics of the human bulbar conjunctiva can be measured easily and reliably using FSLB. The alternations of the fractal dimensions, morphometry and hemodynamics during contact lens wear may indicate ocular microvascular responses to contact lens wear.
•We developed functional slit lamp biomicroscopy (FSLB).•High resolution non-invasive capillary perfusion maps were successfully acquired.•Microvascular network's fractality was automatically segmented and analyzed.•Conjunctival microvascular hemodynamics was simultaneously obtained.•Microvascular alternations were found in contact lens wearers.
