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Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis
Journal article

Growth and recombination of phage lambda in the presence of exonuclease V from Bacillus subtilis

F Stahl, R Bowers, Jr, D Mooney, R Myers, M Stahl and L Thomason
Molecular & general genetics, Vol.264(5), pp.716-723
2001-01
DOI: https://doi.org/10.1007/s004380000361
PMID: 11212927

Abstract

Escherichia coli - enzymology DNA Replication Bacillus subtilis - enzymology Bacteriophage lambda - physiology DNA - metabolism Exodeoxyribonuclease V Plasmids - metabolism Bacillus subtilis - genetics Phenotype Time Factors Escherichia coli - genetics Recombination, Genetic Gene Deletion Exodeoxyribonucleases - physiology Bacteriophage lambda - genetics Exodeoxyribonucleases - genetics Mutation Rec A Recombinases - genetics
When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage lambda. Mutants of lambda that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of lambda ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted.

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