References: Firsov, D., Gautschi, I., Merillat, A.-M., Rossier, B.C., Schild, L., The heterotetrameric architecture of the epithelial sodium channel (ENaC) (1998) EMBO Journal, 17 (2), pp. 344-352. , DOI 10.1093/emboj/17.2.344;
Kelly, O., Lin, C., Ramkumar, M., Saxena, N.C., Kleyman, T.R., Eaton, D.C., Characterization of an amiloride binding region in the-subunit of ENaC (2003) Am. J. Physiol. Renal Physiol., 285, pp. F1279-F1290;
Hummler, E., Rossier, B.C., Physiological and pathophysiological role of the epithelial sodium channel in the control of blood pressure (1996) Kidney and Blood Pressure Research, 19 (3-4), pp. 160-165;
Staub, O., Gautschi, I., Ishikawa, T., Breitschopf, K., Ciechanover, A., Schild, L., Rotin, D., Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination (1997) EMBO Journal, 16 (21), pp. 6325-6336. , DOI 10.1093/emboj/16.21.6325;
Yu, L., Helms, M.N., Yue, Q., Eaton, D.C., Single-channel analysis of functional epithelial sodium channel (ENaC) stability at the apical membrane of A6 distal kidney cells (2008) Am. J. Physiol. Renal Physiol., 295, pp. F1519-F1527;
Lazrak, A., Jurkuvenaite, A., Chen, L., Keeling, K.M., Collawn, J.F., Bedwell, D.M., Matalon, S., Enhancement of alveolar epithelial sodium channel activity with decreased cystic fibrosis transmembrane conductance regulator expression in mouse lung (2011) Am. J. Physiol. Lung Cell Mol. Physiol., 301, pp. L557-L567;
Lazrak, A., Chen, L., Jurkuvenaite, A., Doran, S.F., Liu, G., Li, Q., Lancaster Jr., J.R., Matalon, S., Regulation of alveolar epithelial Na+ channels by ERK1/2 in chlorine-breathing mice (2012) Am. J. Respir. Cell Mol. Biol., 46, pp. 342-354;
Staub, O., Rotin, D., Regulation of ion transport by protein-protein interaction domains (1997) Current Opinion in Nephrology and Hypertension, 6 (5), pp. 447-454;
Kamitani, T., Kito, K., Nguyen, H.P., Yeh, E.T.H., Characterization of NEDD8, a developmentally down-regulated ubiquitin- like protein (1997) Journal of Biological Chemistry, 272 (45), pp. 28557-28562. , DOI 10.1074/jbc.272.45.28557;
Kumar, A., Wu, H., Collier-Hyams, L.S., Kwon, Y.M., Hanson, J.M., Neish, A.S., The bacterial fermentation product butyrate influences epithelial signaling via reactive oxygen species-mediated changes in cullin- 1 neddylation (2009) J. Immunol., 182, pp. 538-546;
Helms, M.N., Jain, L., Self, J.L., Eaton, D.C., Redox regulation of epithelial sodium channels examined in alveolar type 1 and 2 cells patch-clamped in lung slice tissue (2008) J. Biol. Chem., 283, pp. 22875-22883;
Soucy, T.A., Smith, P.G., Milhollen, M.A., Berger, A.J., Gavin, J.M., Adhikari, S., Brownell, J.E., Langston, S.P., An inhibitor of NEDD8- activating enzyme as a new approach to treat cancer (2009) Nature, 458, pp. 732-736;
Goodson, P., Kumar, A., Jain, L., Kundu, K., Murthy, N., Koval, M., Helms, M.N., NADPH oxidase regulates alveolar epithelial sodium channel activity and lung fluid balance in vivo via O signaling (2012) Am. J. Physiol. Lung Cell Mol. Physiol., 302, pp. L410-L419;
Kleyman, T.R., Carattino, M.D., Hughey, R.P., ENaC at the cutting edge. Regulation of epithelial sodium channels by proteases (2009) J. Biol. Chem., 284, pp. 20447-20451;
Rossier, B.C., Stutts, M.J., Activation of the epithelial sodium channel (ENaC) by serine proteases (2009) Annu. Rev. Physiol., 71, pp. 361-379;
Kumar, A., Collier-Hyams, L., Kwon, Y.M., Hanson, J.M., Neish, A.S., Buyrate influences epithelial signaling via generation of reactive oxygen species (2011) FASEB J, 22, p. 328;
Kumar, A., Wu, H., Collier-Hyams, L.S., Hansen, J.M., Li, T., Yamoah, K., Pan, Z.-Q., Neish, A.S., Commensal bacteria modulate cullin-dependent signaling via generation of reactive oxygen species (2007) EMBO Journal, 26 (21), pp. 4457-4466. , DOI 10.1038/sj.emboj.7601867, PII 7601867;
Yu, L., Bao, H.-F., Self, J.L., Eaton, D.C., Helms, M.N., Aldosterone-induced increases in superoxide production counters nitric oxide inhibition of epithelial Na channel activity in A6 distal nephron cells (2007) American Journal of Physiology - Renal Physiology, 293 (5), pp. F1666-F1677. , http://ajprenal.physiology.org/cgi/reprint/293/5/F1666, DOI 10.1152/ajprenal.00444.2006;
Takemura, Y., Goodson, P., Bao, H.F., Jain, L., Helms, M.N., Rac1-mediatedNADPHoxidase release ofO2- regulates epithelial sodium channel activity in the alveolar epithelium (2010) Am. J. Physiol. Lung Cell Mol. Physiol., 298, pp. L509-L520;
Ergonul, Z., Frindt, G., Palmer, L.G., Regulation of maturation and processing of ENaC subunits in the rat kidney (2006) American Journal of Physiology - Renal Physiology, 291 (3), pp. F683-F693. , http://ajprenal.physiology.org/cgi/reprint/291/3/F683, DOI 10.1152/ajprenal.00422.2005;
De La Rosa, D.A., Li, H., Canessa, C.M., Effects of aldosterone on biosynthesis, traffic, and functional expression of epithelial sodium channels in A6 cells (2002) Journal of General Physiology, 119 (5), pp. 427-442. , DOI 10.1085/jgp.20028559;
Xu, H., Chu, S., ENaC α-subunit variants are expressed in lung epithelial cells and are suppressed by oxidative stress (2007) American Journal of Physiology - Lung Cellular and Molecular Physiology, 293 (6), pp. L1454-L1462. , http://ajplung.physiology.org/cgi/reprint/293/6/L1454, DOI 10.1152/ajplung.00248.2007;
Baines, D.L., Albert, A.P., Hazell, M.J., Gambling, L., Woollhead, A.M., Dockrell, M.E., Lipopolysaccharide modifies amiloride-sensitive Na+transport processes across human airway cells. Role of mitogenactivated protein kinases ERK 1/2 and 5 (2010) Pflugers Arch., 459, pp. 451-463;
Tan, C.D., Selvanathar, I.A., Baines, D.L., Cleavage of endogenous -ENaC and elevated abundance of -ENaC are associated with increased Na(+) transport in response to apical fluid volume expansion in human H441 airway epithelial cells (2011) Pflugers Arch., 462, pp. 431-441;
Rafii, B., Tanswell, A.K., Otulakowski, G., Pitkänen, O., Belcastro-Taylor, R., O'Brodovich, H., O2-induced ENaC expression is associated with NF-B activation and blocked by superoxide scavenger (1998) Am. J. Physiol., 275, pp. L764-L770;
Flohé, L., Brigelius-Flohé, R., Saliou, C., Traber, M.G., Packer, L., Redox regulation of NF- B activation (1997) Free Radic. Biol. Med., 22, pp. 1115-1126;
Ismailov, I.I., Awayda, M.S., Berdiev, B.K., Bubien, J.K., Lucas, J.E., Fuller, C.M., Benos, D.J., Triple-barrel organization of ENaC, a cloned epithelial Na+ channel (1996) Journal of Biological Chemistry, 271 (2), pp. 807-816. , DOI 10.1074/jbc.271.2.807;
Stewart, A.P., Haerteis, S., Diakov, A., Korbmacher, C., Edwardson, J.M., Atomic force microscopy reveals the architecture of the epithelial sodium channel (ENaC) (2011) J Biol Chem, 286, pp. 31944-31952;
Stockand, J.D., Staruschenko, A., Pochynyuk, O., Booth, R.E., Silverthorn, D.U., Insight toward epithelial Na+ channel mechanism revealed by the acid-sensing ion channel 1 structure (2008) IUBMB Life, 60, pp. 620-628;
Kabra, R., Knight, K.K., Zhou, R., Snyder, P.M., Nedd4-2 induces endocytosis and degradation of proteolytically cleaved epithelial Na+ channels (2008) J. Biol. Chem., 283, pp. 6033-6039;
Kamynina, E., Staub, O., Concerted action of ENaC, Nedd4-2, and Sgk1 in transepithelial Na(+) transport (2002) Am. J. Physiol. Renal Physiol., 283, pp. F377-F387;
Snyder, P.M., Steines, J.C., Olson, D.R., Relative contribution of Nedd4 and Nedd4-2 to ENaC regulation in epithelia determined by RNA interference (2004) Journal of Biological Chemistry, 279 (6), pp. 5042-5046. , DOI 10.1074/jbc.M312477200;
Zhou, R., Patel, S.V., Snyder, P.M., Nedd4-2 catalyzes ubiquitination and degradation of cell surface ENaC (2007) Journal of Biological Chemistry, 282 (28), pp. 20207-20212. , http://www.jbc.org/cgi/reprint/282/28/20207, DOI 10.1074/jbc.M611329200;
Helms, M.N., Torres-Gonzalez, E., Goodson, P., Rojas, M., Direct tracheal instillation of solutes into mouse lung (2010) J. Vis. Exp., 42, p. 1941;
Gao, D., Wan, L., Inuzuka, H., Berg, A.H., Tseng, A., Zhai, B., Shaik, S., Wei, W., Rictor forms a complex with Cullin-1 to promote SGK1 ubiquitination and destruction (2010) Mol. Cell, 39, pp. 797-808;
Gao, D., Wan, L., Wei, W., Phosphorylation of Rictor at Thr- 1135 impairs the Rictor/Cullin-1 complex to ubiquitinate SGK1 (2010) Protein Cell, 1, pp. 881-885;
Snyder, P.M., Olson, D.R., Kabra, R., Zhou, R., Steines, J.C., cAMP and serum and glucocorticoid-inducible kinase (SGK) regulate the epithelial Na+ channel through convergent phosphorylation of Nedd4-2 (2004) Journal of Biological Chemistry, 279 (44), pp. 45753-45758. , DOI 10.1074/jbc.M407858200;
Asher, C., Sinha, I., Garty, H., Characterization of the interactions between Nedd4-2, ENaC, and sgk-1 using surface plasmon resonance (2003) Biochimica et Biophysica Acta - Biomembranes, 1612 (1), pp. 59-64. , DOI 10.1016/S0005-2736(03)00083-X;
Boehmer, C., Palmada, M., Rajamanickam, J., Schniepp, R., Amara, S., Lang, F., Post-translational regulation of EAAT2 function by coexpressed ubiquitin ligase Nedd4-2 is impacted by SGK kinases (2006) J. Neurochem., 97, pp. 911-921;
Li, T., Koshy, S., Folkesson, H.G., IL-1-induced cortisol stimulates lung fluid absorption in fetal guinea pigs via SGK-mediated Nedd4-2 inhibition (2009) Am. J. Physiol. Lung Cell Mol. Physiol., 296, pp. L527-L533;
Pearce, D., SGK1 regulation of epithelial sodium transport (2003) Cellular Physiology and Biochemistry, 13 (1), pp. 13-20. , DOI 10.1159/000070245;
Snyder, P.M., Olson, D.R., Thomas, B.C., Serum and glucocorticoid-regulated kinase modulates Nedd4-2-mediated inhibition of the epithelial Na+ channel (2002) Journal of Biological Chemistry, 277 (1), pp. 5-8. , DOI 10.1074/jbc.C100623200;
Wiemuth, D., Lott, J.S., Ly, K., Ke, Y., Teesdale-Spittle, P., Snyder, P.M., McDonald, F.J., Interaction of serum- and glucocorticoid regulated kinase 1 (SGK1) with the WW-domains of Nedd4-2 is required for epithelial sodium channel regulation (2010) PLoS ONE, 5, pp. e12163
