Isolation and characterization of cDNA encoding the gamma-subunit of cGMP phosphodiesterase in human retina

Narendra Tuteja; Michael Danciger; Ivana Klisak; Renu Tuteja; George Inana; T Mohandas; Robert S Sparkes; Debora B Farber

doi:10.1016/0378-1119(90)90035-P

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Isolation and characterization of cDNA encoding the gamma-subunit of cGMP phosphodiesterase in human retina

Journal article

Peer reviewed

Isolation and characterization of cDNA encoding the gamma-subunit of cGMP phosphodiesterase in human retina

Narendra Tuteja, Michael Danciger, Ivana Klisak, Renu Tuteja, George Inana, T Mohandas, Robert S Sparkes and Debora B Farber

Gene, Vol.88(2), pp.227-232

1990

DOI: https://doi.org/10.1016/0378-1119(90)90035-P

PMID: 2161380

Abstract

sequencing mRNA

chromosome assignment

molecular cloning

Recombinant DNA

photoreceptor degeneration

deduced amino acid sequence

somatic cell hybrids

in situ hybridization

Cyclic GMP-phosphodiesterase (cGMP-PDE) plays a key role in the normal functioning of retinal rod photoreceptor cells. The enzyme is composed of α- and β-catalytic which are inhibited by two identical γ-subunits. A cDNA encoding the γ-subunits (PDEγ) from human retina has been cloned and sequenced. The 1012-bp cDNA has a coding region of 261 bp which is high homologous to those of the PDEγcDNAs from bovine and mouse retinas. Comparison of the deduced amino acid sequences of the proteins from the three species indicates that PDEγ has been very well conserved through evolution. The mRNA encoded by the cloned cDNA is 1.0 kb long, is similar in size to the corresponding mRNAs from mouse, dog and bovine retinas and is not detected in ground squirrel retina. The PDEG gene has been assigned to human chromosome 17, probably in the region q21.1.

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.36 Ophthalmology; 1.36.212 Retina
Web Of Science research areas: Genetics & Heredity
ESI research areas: Molecular Biology & Genetics

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Details

Title: Isolation and characterization of cDNA encoding the gamma-subunit of cGMP phosphodiesterase in human retina
Creators: Narendra Tuteja - Jules Stein Eye Institute and Department of Ophthalmology, UCLA School off Medicine, Los Angeles, CA 90024 U.S.A. Tel 206-6800
Michael Danciger - Jules Stein Eye Institute and Department of Ophthalmology, UCLA School off Medicine, Los Angeles, CA 90024 U.S.A. Tel 206-6800
Ivana Klisak - Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90024 U.S.A. Tel. 825-5720
Renu Tuteja
George Inana - Bascom Palmer Eye Institute, University of Miami School of Medicine, Miami, FL 33136 U.S.A. Tel. 326-6031
T Mohandas - Division of Medical Genetics, Harbor/UCLA Medical Center, Torrance, CA 90509 U.S.A. Tel. 533-3764
Robert S Sparkes - Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90024 U.S.A. Tel. 825-5720
Debora B Farber - Jules Stein Eye Institute and Department of Ophthalmology, UCLA School off Medicine, Los Angeles, CA 90024 U.S.A. Tel 206-6800
Publication Details: Gene, Vol.88(2), pp.227-232
Publisher: Elsevier B.V
Academic Unit: Miller School of Medicine; UMMG Department of Ophthalmology
Language: English
Resource Type: Journal article
PMID: 2161380
Record Identifier: 991031562107602976