Isolation, structural elucidation and synthesis of a tetradecapeptide with in vitro ACTH-releasing activity corresponding to residues 33–46 of the α-chain of porcine hemoglobin

A.V Schally; W.Y Huang; T.W Redding; A Arimura; D.H Coy; K Chihara; R.C.C Chang; V Raymond; F Labrie

doi:10.1016/0006-291X(78)90914-2

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Isolation, structural elucidation and synthesis of a tetradecapeptide with in vitro ACTH-releasing activity corresponding to residues 33–46 of the α-chain of porcine hemoglobin

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Isolation, structural elucidation and synthesis of a tetradecapeptide with in vitro ACTH-releasing activity corresponding to residues 33–46 of the α-chain of porcine hemoglobin

A.V Schally, W.Y Huang, T.W Redding, A Arimura, D.H Coy, K Chihara, R.C.C Chang, V Raymond and F Labrie

Biochemical and biophysical research communications, Vol.82(2), pp.582-588

1978

DOI: https://doi.org/10.1016/0006-291X(78)90914-2

PMID: 208567

Abstract

A peptide found in acetic acid extracts of porcine hypothalami and capable of stimulating the release of ACTH in vitro was isolated in pure state, structurally identified as Phe-Leu-Gly-Phe-Pro-Thr-Thr-Lys-Thr-Tyr-Pre-Pro-His-Phe and synthesized. This tetradecapeptide, which corresponds to amino acid residues no. 33–46 in the sequence of the α-chain of porcine hemoglobin, probably represents an artefact of extraction or isolation procedures. Since this peptide stimulates ACTH release from rat pituitary fragments and from monolayer cultures of pituitary cells, but not in vivo , caution must be exercised in interpreting the results of in vitro assays for corticotropin releasing factor.

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Title: Isolation, structural elucidation and synthesis of a tetradecapeptide with in vitro ACTH-releasing activity corresponding to residues 33–46 of the α-chain of porcine hemoglobin
Creators: A.V Schally - V.A. Hospital, New Orleans, LA 70146 USA
W.Y Huang - V.A. Hospital, New Orleans, LA 70146 USA
T.W Redding - V.A. Hospital, New Orleans, LA 70146 USA
A Arimura - V.A. Hospital, New Orleans, LA 70146 USA
D.H Coy - V.A. Hospital, New Orleans, LA 70146 USA
K Chihara - V.A. Hospital, New Orleans, LA 70146 USA
R.C.C Chang - V.A. Hospital, New Orleans, LA 70146 USA
V Raymond - V.A. Hospital, New Orleans, LA 70146 USA
F Labrie - V.A. Hospital, New Orleans, LA 70146 USA
Publication Details: Biochemical and biophysical research communications, Vol.82(2), pp.582-588
Publisher: Elsevier Inc
Academic Unit: Miller School of Medicine; UMMG Department of Pathology
Language: English
Resource Type: Journal article
PMID: 208567
Record Identifier: 991031613503002976