Abstract
Aim:
Mitochondrial cytochrome
c
oxidase (COX), the last enzyme of the respiratory chain, catalyzes the reduction of oxygen to water and therefore is essential for cell function and viability. COX is a multimeric complex, whose biogenesis is extensively regulated. One type of control targets cytochrome
c
oxidase subunit 1 (Cox1), a key COX enzymatic core subunit translated on mitochondrial ribosomes. In
Saccharomyces cerevisiae
, Cox1 synthesis and COX assembly are coordinated through a negative feedback regulatory loop. This coordination is mediated by Mss51, a heme-sensing
COX1
mRNA-specific processing factor and translational activator that is also a Cox1 chaperone. In this study, we investigated whether Mss51 hemylation and Mss51-mediated Cox1 synthesis are both modulated by the reduction–oxidation (redox) environment.
Results:
We report that Cox1 synthesis is attenuated under oxidative stress conditions and have identified one of the underlying mechanisms. We show that
in vitro
and
in vivo
exposure to hydrogen peroxide induces the formation of a disulfide bond in Mss51 involving CPX motif heme-coordinating cysteines. Mss51 oxidation results in a heme ligand switch, thereby lowering heme-binding affinity and promoting its release. We demonstrate that in addition to affecting Mss51-dependent heme sensing, oxidative stress compromises Mss51 roles in
COX1
mRNA processing and translation.
Innovation:
H
2
O
2
-induced downregulation of mitochondrial translation has so far not been reported. We show that high H
2
O
2
concentrations induce a global attenuation effect, but milder concentrations specifically affect
COX1
mRNA processing and translation in an Mss51-dependent manner.
Conclusion:
The redox environment modulates Mss51 functions, which are essential for regulation of COX biogenesis and aerobic energy production.
Antioxid. Redox Signal.
24, 281–298.
