Protocol to study regeneration in human pancreatic slices using viral tracing, extended culture, dissociation, and functional Ca 2+ influx techniques

This work was funded by the Diabetes Research Institute Foundation (DRIF), Tonkinson Foundation, the NIH-sponsored Integrated Islet Distribution Program (IIDP), and NIH grants 1R01DK130846-01 (J.D-B./R.P.), 5R01DK116875-05 (J.D-B./R.P.), U01DK120393 (J.D-B./R.P.) and K99DK140067-01A1 (M.M.F.Q.). It was also supported by the Network for Pancreatic Organ Donors with Diabetes (nPOD; RRID:SCR_014641), a collaborative type 1 diabetes research project sponsored by Breakthrough T1D (nPOD: 5-SRA-2018-557-Q-R) and The Leona M. & Harry B. Helmsley Charitable Trust (grant#2018PG-T1D053), as well as The Helmsley Charitable Trust George S. Eisenbarth nPOD award for Team Science (2015-PG-T1D-052). The content and views expressed are the responsibility of the authors and do not necessarily reflect the official view of nPOD. Organ Procurement Organizations (OPOs) partnering with nPOD to provide research resources are listed at http://www.jdrfnpod.org//for-partners/npod-partners/. Finally, we wholeheartedly thank the donors and their families for their invaluable contribution to science. Drs. J.D-B. and R.P. are the guarantors of this work and, as such, had full access to all the data and take responsibility for the integrity and the accuracy of the analysis.