Abstract
Research rigor can be enhanced by pairing genetic tools with pharmacology and manipulations of solutes or ions. Here, we present a protocol for treating
C. elegans
with pharmacological agents, osmoles, and salts. We describe steps for agar plate supplementation, addition of the compound to the polymerized plates, and using liquid culture for exposure to the chemical. Treatment type depends on the stability and solubility of each compound. This protocol is applicable to both behavioral and
in vivo
imaging experiments.
For complete details on the use and execution of this protocol, please refer to Wang et al. (2022),
1
Fernandez-Abascal et al. (2022),
2
and Johnson et al. (2020).
3
•
Protocols for treating
C. elegans
with ions, solutes, and pharmacological agents
•
Compounds are added to the agar before or after polymerization
•
Pharmacological agents can be added to liquid culture
•
Protocols can be used for imaging and behavioral assays
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Research rigor can be enhanced by pairing genetic tools with pharmacology and manipulations of solutes or ions. Here, we present a protocol for treating
C. elegans
with pharmacological agents, osmoles, and salts. We describe steps for agar plate supplementation, addition of the compound to the polymerized plates, and using liquid culture for exposure to the chemical. Treatment type depends on the stability and solubility of each compound. This protocol is applicable to both behavioral and
in vivo
imaging experiments.