Abstract
The binding kinetics of fluorescently labeled microtubules to kinesin‐coated surfaces permits the determination of the density of kinesin motor proteins adhered to the surface in the range of 0.1 – 30 μm–2. This extreme sensitivity, corresponding to protein coverages of 0.004 – 1 ng cm–2, enables the characterization of advanced non‐fouling coatings, such as (EG)3OH‐terminated SAMs and PEGMA with applications in biomedical engineering and bionanotechnology.
