Abstract
Evidence is presented that
Giardia lamblia and
Entamoeba histolytica, phylogenetically unrelated aerotolerant anaerobes, have crucial thiol groups on or easily accessible to their external surface. Both parasites were killed by three structurally unrelated thiol-blocking reagents which penetrate intact cells poorly or not at all. The parasites were protected from
p-chloromercuribenzenesulfonic acid (10–100 μM) by cysteine or by reduced glutathione. Killing was arrested with identical kinetics by addition of either cysteine (which quickly penetrates the cells) or bovine serum albumin (which does not penetrate intact cells) at various times
after
p-chloromercuribenzenesulfonic acid, indicating that the reactive site may be on the outer surface of the cell. Proteins lacking cysteine did not protect. Sensitivity of three other protozoa to
p-chloromercuribenzenesulfonic acid was also tested.
Trichomonas vaginalis (anaerobic) was at least as sensitive as
E. histolytica and
G. lamblia, while
Crithidia fasciculata and
Paramecium tetraurelia (both aerobic) were less sensitive. Thiol groups on the
G. lamblia surface were demonstrated directly by fluorescence-activated cell sorter analysis of trophozoites which had been modified with a thiol-specific hapten,
N-iodoacetyl-
N-(5-sulfonic-1-naphthyl)ethylenediamine and reacted with fluorescent antibody to this hapten.
