Abstract
Gel electrophoresis in the presence of sodium dodecyl sulphate followed by electroblotting was employed in sample preparation for microsequencing proteins and protein fragments. Three types of solid supports were compared: glass fiber filters modified by aminopropyltriethoxysilane or covered with polybrene, and polyvinylidenedifluoride membranes. N-Terminal amino acid sequences of several proteins (Mr 14-140 kDA were determined on a gas-phase sequencer with the standard programme; 20-200 pmoles of the protein can be assayed by this method.
